BME100 f2015:Group5 8amL4: Difference between revisions

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| [[Image:BME103student.jpg|100px|thumb|Name: Tyler Mitchell<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]]
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'''Materials'''
'''Materials'''
<!-- Record all of the materials you will need for PCR -->
<!-- Record all of the materials you will need for PCR -->
*a. Lab coat and disposable gloves
* Item 1
*b. PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2
* Item 2
, and dNTP’s
* etc.
*c. DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes
have the same forward primer and reverse primer
*d. A strip of empty PCR tubes
*e. Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or
samples will be cross-contaminated
*f. Cup for discarded tips
*g. Micropipettor
*h. OpenPCR machine: shared by two groups
 




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<br><br>
<br><br>
==SNP Information & Primer Design==
'''Background: About the Disease SNP'''
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. -->
'''Primer Design and Testing'''
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. -->




Revision as of 08:36, 14 October 2015

BME 100 Fall 2015 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: student
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LAB 4 WRITE-UP

Protocol

Materials

  • Item 1
  • Item 2
  • etc.


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G# + Positive control none
G# - Negative control none
G# 1-1 Patient 1, replicate 1
G# 1-2 Patient 1, replicate 2
G# 1-3 Patient 1, replicate 3
G# 2-1 Patient 2, replicate 1
G# 2-2 Patient 2, replicate 2
G# 2-3 Patient 2, replicate 3


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program






Research and Development

PCR - The Underlying Technology






SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing




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