BME100 f2016:Group14 W8AM L5: Difference between revisions
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<!-- INSTRUCTIONS: In the space below, describe in detail how your team set up your device to capture images from the fluorimeter. --> | <!-- INSTRUCTIONS: In the space below, describe in detail how your team set up your device to capture images from the fluorimeter. --> | ||
To capture images from the flourimeter first the flourimeter was set up by turning it on andplacing a slide in with the smooth side down. We then, set up a phone timer to 3 seconds and turned off the flash. The phone went into a craddle and the flourimeter was lifted with boxes until the slide was in horizontal view of the camera. | |||
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<!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter --> | <!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter --> | ||
# '' | # ''Set micropippete to 80 uL'' | ||
# '' | # ''Place tip on micropipette'' | ||
# '' | # ''Press down on micropipette to first stop'' | ||
# '' | # ''Place tip to SYBR Green I solution and release to pick up liquid'' | ||
# ''Place tip between first 2 circles in the middle of the glass slide'' | |||
# ''Press down to second stop on micropipette to release liquid onto slide'' | |||
# ''Dispose of tip'' | |||
# ''Place new tip on micropipette'' | |||
# ''Press down on miropipette to first stop'' | |||
# ''Place tip to sample/calibration solution and release to pick up liquid'' | |||
# ''Place tip onto drop of SYBR Green I drop'' | |||
# ''Press down to second stop to release the sample/calibration drop on top of the SYBR Green I drop'' | |||
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Revision as of 09:19, 26 October 2016
BME 100 Fall 2016 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportThe team's experience went very well with pipetting. Everyone in the team got to pipette and familiarize themselves by the end of the period. Yes, the pre-lab reading helped. The first stop is to pick up the solution and the second stop is to expel it. Yes, the final reactions had the same amount of liquid and there was not any liquid left in the tubes. We kept our labeling scheme the same. Fluorimeter ProcedureImaging set-up To capture images from the flourimeter first the flourimeter was set up by turning it on andplacing a slide in with the smooth side down. We then, set up a phone timer to 3 seconds and turned off the flash. The phone went into a craddle and the flourimeter was lifted with boxes until the slide was in horizontal view of the camera.
Placing Samples onto the Fluorimeter
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
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