BME100 f2016:Group16 W8AM L5

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Nandini Sharma Name: student Name: student Name: student Name: student Name: student LAB 5 WRITE-UP PCR Reaction Report For Part C of the lab, our group prepped our samples for the Polymerase Chain Reaction. To complete this we needed to know proper lab procedure such as how to pipette and label. The pre-lab was helpful in informing us of the steps in the process of pipetting. The first step was to label the tubes. We were able to maintain the labeling structure that we established in the initial parts of this lab. Once we had the pipette it was easy to fell the difference between the first and second stop. It takes more force to get to the second stop than it does to get to the first stop. While pipetting, it was difficult to ensure that all the liquid was all collected. It took a few tries on the first few tubes to ensure all the liquid was empties. By the end we were able to ensure that majority of the 50 microliters of the PCR mix and each sample were pipetted into the tubes. The overall process was tedious because of the amount of time it took but it was fairly simple and easy to understand. Fluorimeter Procedure Imaging set-up When setting up our device to capture the images, we placed the phone, which in this case was an iPhone 7, in an upright position focused on the drop. We placed the phone approximately 7cm away from the drop and used a timer to capture the image. We used an eraser to help hold it in position while it was in the stand and then we used a folder to cover the back of the fluorimeter to keep all of the light out. Placing Samples onto the Fluorimeter [Instructions: Step one, in your own words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :