BME100 f2016:Group16 W8AM L5
BME 100 Fall 2016 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportFor Part C of the lab, our group prepped our samples for the Polymerase Chain Reaction. To complete this we needed to know proper lab procedure such as how to pipette and label. The pre-lab was helpful in informing us of the steps in the process of pipetting. The first step was to label the tubes. We were able to maintain the labeling structure that we established in the initial parts of this lab. Once we had the pipette it was easy to fell the difference between the first and second stop. It takes more force to get to the second stop than it does to get to the first stop. While pipetting, it was difficult to ensure that all the liquid was all collected. It took a few tries on the first few tubes to ensure all the liquid was empties. By the end we were able to ensure that majority of the 50 microliters of the PCR mix and each sample were pipetted into the tubes. The overall process was tedious because of the amount of time it took but it was fairly simple and easy to understand. Fluorimeter ProcedureImaging set-up
Placing Samples onto the Fluorimeter
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
|