BME100 s2014:T Group15 L5

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Ariana L. Shults Name: Samantha J Hatley Name: Megan Mendieta Name: Heston Scott Hamilton Name: Mohammed A. Tamim Name: Brenda Cardenas LAB 5 WRITE-UP Background Information SYBR Green Dye [Instructions: A short summary describing SYBR green dye] Single-Drop Fluorimeter [Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] How the Fluorescence Technique Works [Instructions: In your own words] Procedure Smart Phone Camera Settings [Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: [Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part] Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = [Instructions: See worksheet page 6.] Solutions Used for Calibration [Instructions: See worksheet page 6.] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 [Add more rows as needed] Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples PCR Product TUBE LABEL Concentration Volume of the DILUTED PCR Product solution (µL) Volume of the SYBR GREEN I Dye solution (µL) INTDENS VALUES BASED ON 3 SEPARATE DROP MEASUREMENTS ' ' AVERAGE INTDENS VALUE Corrected PCR Concentration 1. 2.5 Calf Thymus 2.25 80 80 7298707 5868419 6324719 6497281.7 27.00 2. 0.5 Calf Thymus 0.50 80 80 2282276 2152201 3068249 2500908.7 6.00 3. 0.25 Calf Thymus 0.25 80 80 1730534 2262858 2032111 2008501.0 3.00 4. 0 Calf Thymus 0.00 80 80 1464326 1440224 1654233 1519594.3 0.00 5. + (DNA) 1.77 80 80 4796701 4114399 4693591 4534897.0 21.21 6. P11 (DNA) 0.21 80 80 1310944 1260384 1673944 1415090.7 2.49 7. P12 (DNA) 0.59 80 80 1459086 3138435 1942214 2179911.7 7.08 8. P13 (DNA) 0.47 80 80 1793887 1849966 2158787 1934213.3 5.61 9. - (DNA) 0.26 80 80 1461537 2052869 1075174 1529860.0 3.18 10. P21 (DNA) 0.39 80 80 1915305 1464238 1974133 1784558.7 4.71 11. P22 (DNA) 0.14 80 80 1051004 1464473 1296561 1270679.3 1.62 12. P23 (DNA) 0.46 80 80 1927895 2326738 1528947 1927860.0 5.57 Fitting a Straight Line [Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]