BME100 s2014:T Group2 L6
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
THE CR3W
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD In the beginning each part of the PCR machine was imported to Tinkercad. The files represented were 'Body: Back, Bottom, Side, Side with ports, and Top.
[Instructions: A short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design?]
Feature 1: Disease SNP-Specific Primersrs237025 is a SNP(Single nucleotide polymorphism) mainly found in Homo Sapiens. Meaning Humans are most often exhibit this variation. A nucleotide is basic unit of nucleic acid. A polymorphism is The presence of two or more phenotypes in a population because of the expression of different alleles of a given gene. rs237025 is seen on the 6:149721690 chromosome. Normally, when disease of classified, they fall under a certain clinical significance. Basically its general effect on day-to-day. That significance could be palpable, real genuine, or a noticeable effect. rs237025 did not fit any of these categories. Diseases closely linked to rs237025 are diabetes and Vogt-Koyanagi-Harada syndrome, a multi-system disease with dermatological effects.
Primer design
How the primers work: Primers are single strands of DNA that act as the beginning of DNA synthesis. Primers are set to the beginning and end of the desired DNA fragment that will be amplified. Thus this will keep it from amplifying any non-disease alleles.
Feature 2: Consumables Kit[Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.] [Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph.]
Feature 3: Hardware - PCR Machine & Fluorimeter[Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.]
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]
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