BME100 s2014:T Group8 L5: Difference between revisions
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| [[Image:ColtonTucker.jpg|100px|thumb|Name: Colton Tucker]] | | [[Image:ColtonTucker.jpg|100px|thumb|Name: Colton Tucker]] | ||
| [[Image:Henrykim.jpg|100px|thumb|Name: Hooyoung Kim]] | | [[Image:Henrykim.jpg|100px|thumb|Name: Hooyoung Kim]] | ||
| [[Image: | | [[Image:OpenwetwarefillerCallowayGroup8.jpg|100px|thumb|Name: Callaway Freeland]] | ||
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'''Solutions Used for Calibration'''<br> | |||
{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
! colspan="3" | Distance of Phone from Fluorimeter | |||
| 6.5 cm | |||
|- | |- | ||
| | ! Initial Concentration of 2X Calf Thymus DNA solution (μg/mL) | ||
! Volume of the 2X DNA solution (μL) | |||
! Volume of the SYBR Green I Dye Solution | |||
! Final DNA concentration in SYBR Green I Solution (μg/mL) | |||
|- | |||
| 5.0 | |||
| 80 | |||
| 80 | |||
| 2.5 | |||
|- | |||
| 2.0 | |||
| 80 | |||
| 80 | |||
| 1.0 | |||
|- | |||
| 1.0 | |||
| 80 | |||
| 80 | |||
| 0.5 | |||
|- | |||
| 0.5 | |||
| 80 | |||
| 80 | |||
| 0.25 | |||
|- | |||
| 0.25 | |||
| 80 | |||
| 80 | |||
| .0125 | |||
|- | |||
| 0.0 | |||
| 80 | |||
| 80 | |||
| 0.0 | |||
|- | |- | ||
|} | |} | ||
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{| | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
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[[Image:Lab5graphGROUP8.png|600x750px|center|Intends values for each Calf Thymus DNA concentration.]]<br> | [[Image:Lab5graphGROUP8.png|600x750px|center|Intends values for each Calf Thymus DNA concentration.]]<br> | ||
Intends values for each Calf | Intends values for each Calf Thymus DNA cocnentration. <br> | ||
<br> | <br> | ||
'''PCR Results Summary'''<br> | '''PCR Results Summary'''<br> | ||
A Polymerase Chain Reaction (PCR) was completed for 8 DNA samples--3 DNA samples from each of two patients and a positive and a negative control sample. After the reactions and cool down, the samples were tested on a fluorimeter for amplification of DNA using SYBR Green I as a stain. The controls were necessary to ensure amplification results for the patients' samples had a comparative standard and the results were due to the procedure and not an external affect. Using known concentration of DNA from the calibration phase, a standard curve was created that was a basis for conversion from imageJ INTEN values to DNA concentrations for the PCR samples. <br> | |||
Your positive control PCR result was | Your positive control PCR result was 90.744 μg/mL <br> | ||
Your negative control PCR result was | Your negative control PCR result was -2.770 μg/mL <br> | ||
Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed <br> | Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed <br> |
Revision as of 20:59, 16 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
The camera phone was set up in the cradle with an angle to view the side of the drop. The fluorimeter was raised about 1 1/4 inches to accomodate for the phone height.
The phone was removed from this image to simplify and completely show distance setup.
Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples High DNA - using green channel
[Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
Intends values for each Calf Thymus DNA cocnentration. PCR Results Summary A Polymerase Chain Reaction (PCR) was completed for 8 DNA samples--3 DNA samples from each of two patients and a positive and a negative control sample. After the reactions and cool down, the samples were tested on a fluorimeter for amplification of DNA using SYBR Green I as a stain. The controls were necessary to ensure amplification results for the patients' samples had a comparative standard and the results were due to the procedure and not an external affect. Using known concentration of DNA from the calibration phase, a standard curve was created that was a basis for conversion from imageJ INTEN values to DNA concentrations for the PCR samples. Your positive control PCR result was 90.744 μg/mL Write-in each patient ID and give both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed
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