BME100 s2014:T Group9 L4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
# 1. Collect the following items: 8 empty PCR tubes, 8 PCR tubes filled with PCR liquid, the negative control, the positive control, and the 6 PCR tubes filled with samples from the patients. | # 1. Collect the following items: 8 empty PCR tubes, 8 PCR tubes filled with PCR liquid, the negative control, the positive control, and the 6 PCR tubes filled with samples from the patients. | ||
# | # 2. Cut the PCR tubes so they are in groups of 4. | ||
# | # 3. Label the side of the tubes with 8 different predetermined labels. | ||
# 4. Put labeled tubes back in rack. | |||
# 5. Using proper pipetting technique, transfer 50 μL of PCR reaction mix into each of the 8 labeled tubes. | |||
# 6. In each of the labeled tubes, transfer the given DNA into the tube labeled for that DNA. Make sure to use a fresh pipette tip every single time! | |||
# 7. Close all the lids tightly. | |||
# 8. Take tubes to assigned PCR machine and wait for TAs to help, | |||
Revision as of 22:37, 2 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine's LCD display turned off. When we unplugged the white wire that connects (part 6) to (part 2), the machine the temperature reading showed as -40 degrees Celsius and showed a wrong reading.
On March 10th, 2014 between the hours of 9am and 12pm, the test run was attempted. 12 runs were completed and the machine passed.
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology
Finding the Disease-Associated Sequence: Designing a Disease Sequence-Specific Primer Pair:
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