BME100 s2014:T Group9 L5: Difference between revisions
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''[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]'' | ''[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]'' | ||
* Distance between the smart phone cradle and drop = | * Distance between the smart phone cradle and drop = 8 cm | ||
''[Instructions: See worksheet page 6.]'' | ''[Instructions: See worksheet page 6.]'' | ||
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==Data Analysis== | ==Data Analysis== |
Revision as of 21:15, 16 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye SYBR green dye is used as a nucleic acid stain and binds to DNA. The result is a DNA-dye-complex that absorbs blue light and emits green light. This dye can also bind to single-stranded DNA and RNA with a lower performance.
A single-drop fluorimeter is made up of a sturdy black cover used to block out the light. There is a phone cradle used o hold the camera while taking pictures. Inside the black cover there is a platform to hold the slides. There is also an LED light placed around the slides. This light emits a blue light used to see the SYBR green dye.
How the Fluorescence Technique Works The blue LED light shines on the DNA-dye samples. The amount of DNA in each sample is measured based on the concentration of the green light being emitted from the sample.
ProcedureSmart Phone Camera Settings
[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples Tube A Tube B Tube C Tube D Tube E Tube F Tube G Tube H 2308221 14630361 4130155 11153155 5325157 5282577 17579974 22388687 4078569 16780232 4773563 13454922 5753055 5069909 20260575 21671092 4107249 17148850 5055747 11018266 7644472 5291947 18268541 21359871
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