BME100 s2014:T Group9 L6

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Cassaundra Kinnear Name: Katherine Underwood Name: Kyle Queen Name: Mohammed Binsheliail [Instructions: add the name of your team's company and/or product here] LAB 6 WRITE-UP Computer-Aided Design TinkerCAD [Instructions: A short summary (up to five sentences) of the TinkerCAD tool and how you used it in lab on November 20th] Our Design [Instructions: Show an image of your TinkerCAD design here] [Instructions: A short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design?] Feature 1: Disease SNP-Specific Primers [Instructions: This information will come from the exercises you did in PCR Lab B.] Background on the disease-associated mutation [Instructions: Use the answers from questions 3 - 7 to compose, in your own words, a paragraph about rs237025] Primer design Disease SNP-specific Forward Primer: [Instructions: type the sequence of the forward primer] Reverse Primer: [Instructions: type the sequence of the reverse primer] How the primers work: After separating the DNA from the double helix design into single strands, the primer binds with the DNA if and only if the primer and the existing DNA are complementary. They will continue to replicate at this point. If they are not complementary, they will not replicate because the DNA and primer cannot replicate with a different set. Feature 2: Consumables Kit The consumables kit will be held within a small b ox. This will include all the materials needed to preform a small number of PCR reactions. All items will be packaged in groups based on the item. They will then be labeled with a short description, and then they will be placed in the consumables box. This includes 1. Variety of Tubes, including the PCR tubes 2. SYBR Green I sample 3. Micro Pipette Tips 4. Primer sample 5. Micro-pipette 6. Tray to hold tubes Feature 3: Hardware - PCR Machine & Fluorimeter In order to create an easier teaching environment, the current PCR design has been revamped to include a clear lid. The lid will consist of both glass and plastic. The glass will be facing the samples on the inside of the lid in order to keep the samples safe from outside contaminants and regulate the temperature, and heavy duty plastic will surround the glass to give more stability to the machine. Both the PCR machine and Fluorimeter will be packaged in separate boxes for clarity and then placed in a larger box, along with the consumables kit. They will both come dismantled but this will include an easy to follow, picture instructions to help users easily mantle the PCR machine and Fluorimeter. Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.] When looking into the PCR results, it was made clear that the machine did not do the best job at predicting illness. For the percentage of people who developed the disease and also received a positive result from the machine was not a strong percentage. Unfortunately, it was not close to one, like it ideally would be. On the other hand, the people who did not develop the illness and received a negative result was closer to 1 than the prior results. While that is a much stronger result, overall the machine does not have a strong reliability.