BME100 s2015:Group8 9amL4

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: An Tran Role(s) Name: Molly Golek Role(s) Name: Anthony Gambirazio Role(s) Name: Austin Lehew Role(s) Name: Robert Culibrk Role(s) LAB 4 WRITE-UP Protocol Materials Lab Coat Disposable Gloves PCR Reaction Mix, 8 tubes, 50 uL each: Mix contatins Taq DNA polymerase, MgCL2, and dNTP's DNA/primer mix, 8 tubes, 50 uL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or samples will be cross-contaminated Cup for discarded tips Micropipettor OpenPCR machines: shared by two groups PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G8 P Positive control none G8 N Negative control none G8 1-1 Patient 1, replicate 1 G8 1-2 Patient 1, replicate 2 G8 1-3 Patient 1, replicate 3 G8 2-1 Patient 2, replicate 1 G8 2-2 Patient 2, replicate 2 G8 2-3 Patient 2, replicate 3 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Research and Development PCR - The Underlying Technology