BME100 s2016:Projects5

BME 100 Spring 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
Lab 5: “PCR and Fluorescence Detection of DNA” Content from PCR Lab C and Lab D PCR Reactions Procedure Data Analysis HW: Lab Report 5 (due 11/03/15 at 11:59 pm through OpenWetWare) Grading Scheme: 10 points – Overall layout: Our Team section, grammar, style 10 points - Lab C content - PCR reaction report 15 points – Lab D content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter 15 points – Lab D content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary 50 points – Peer Assessment Rubric     EXCELLENT ACCEPTABLE WEAK* OVERALL LAYOUT OUR TEAM section 5 pts = Original & non-offensive images; real names 3 pts = More than half original images & information 1 pt = All default green & white avatars; default text Grammar & style 5 pts = Composition & layout perfect throughout 3 pts = Few spelling errors; images too big or small 1 pt = Many errors PCR REACTION REPORT Summary 10 pts = Complete description, good text formatting 5 pts = Poor composition 1 pt = Missing (only default text) FLUORIMETER PROCEDURE Camera settings 5 pts = Type of smartphone; settings where applicable, or "N/A" 3 pt = Brand name only; vague 1 pts = Missing all info Camera set-up 5 pts = Clear description; distance btwn. camera & drop included 3 pts = Missing one part of the information 2 pts = Only one sentence, missing distance Placing samples onto the fluorimeter 5 pts = Steps are thorough, clear, and easy to follow 3 pts = Run-on paragraphs 1 pt = Instructions are vague/ incomplete DATA COLLECTION & ANALYSIS Callibration: 3 drop images, data table, 2 plots with best fit lines 7 pts = Complete 5 pts = Missing one part, somewhat unorganized 3 pts = Missing two or more parts, very unorganized PCR results: 2 drop images, data table, final results 8 pts = Complete 6 pts = Missing one part, somewhat unorganized 4 pts = Missing two or more parts, very unorganized Note: *Empty/ plagiarized sections or borrowed images without references receive zero points Important Information About Grading: Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited. Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline. Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT! If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button. This should open up an Edit page that is pre-populated with a lab report template code. <createbox> preload=Template:ASUBME103_f2015_L5 prefix=BME100_f2015: width=25 buttonlabel=Create Page </createbox> Group1_8amL5 Group2_8amL5 Group3_8amL5 Group4_8amL5 Group5_8amL5 Group6_8amL5 Group7_8amL5 Group8_8amL5 Group9_8amL5 Group10_8amL5 Group11_8amL5 Group12_8amL5 Group13_8amL5 Group14_8amL5 Group15_8amL5 Group16_8amL5 Group17_8amL5   Group1_1030amL5 Group2_1030amL5 Group3_1030amL5 Group4_1030amL5 Group5_1030amL5 Group6_1030amL5 Group7_1030amL5 Group8_1030amL5 Group9_1030amL5 Group10_1030amL5 Group11_1030amL5 Group12_1030amL5 Group13_1030amL5 Group14_1030amL5 Group15_1030amL5 Group16_1030amL5 Group17_1030amL5   GroupTA_L5 LAB 5 WRITE-UP PCR Reaction Report Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Camera set-up Distance between the smart phone cradle and drop = Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :