BME100 s2017:Group1 W8AM L4: Difference between revisions
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What is the function of each component of a PCR reaction? | What is the function of each component of a PCR reaction? | ||
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What happens to the components (listed above) during each step of thermal cycling? <br> | What happens to the components (listed above) during each step of thermal cycling? <br> | ||
Initial step (95°C for 3 minutes): It makes DNA double helix separates and activate the hot start polymerase. <br> | Initial step (95°C for 3 minutes): It makes DNA double helix separates and activate the hot start polymerase. <br> | ||
Denature (95°C for 30 seconds): The template DNA double helix separates,creating two single-stranded DNA molecules. <br> | Denature (95°C for 30 seconds): The template DNA double helix separates,creating two single-stranded DNA molecules. <br> | ||
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DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below? <br> | DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below? <br> | ||
Adenine (A): Thymine (T) <br> | Adenine (A): Thymine (T) <br> | ||
Thymine (T): Adenine (A) <br> | Thymine (T): Adenine (A) <br> | ||
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During which two steps of thermal cycling does base-pairing occur? <br> | During which two steps of thermal cycling does base-pairing occur? <br> | ||
Annealing and Extending are the two steps of the thermal cycling in which the base-pairing occurs. In the annealing step, when the temperature drops, the DNA is separated in two strand which allows the primers to attach to it. After, in the extending step, the temperature goes up to 72 degrees which allows the DNA polymerase to pair the correct bases to the DNA strand. | Annealing and Extending are the two steps of the thermal cycling in which the base-pairing occurs. In the annealing step, when the temperature drops, the DNA is separated in two strand which allows the primers to attach to it. After, in the extending step, the temperature goes up to 72 degrees which allows the DNA polymerase to pair the correct bases to the DNA strand. | ||
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Latest revision as of 22:37, 21 March 2017
BME 100 Spring 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||
THAT BME GROUPLAB 4 WRITE-UPProtocolMaterials
DNA Sample Set-up Procedure
Research and DevelopmentPCR - The Underlying Technology What is the function of each component of a PCR reaction?
SNP Information & Primer DesignBackground: About the Disease SNP
The disease forward primer changes the last letter of that primer, resulting in
Primer Design and Testing
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