BME100 s2017:Group2 W8AM L5

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

PCR Reaction Report

The PCR pipetting experience went well because our designated pipettor had previous experience using a micropipettor. The pre-lab reading was a good refresher and helped everyone to better understand micropipetting. The difference between the first and the second stop on the pipettor was clear after some practice. The final amount of liquids in the final PCR tubes was the same, but the amount left in the PCR reaction mix and DNA sample tubes varied a little. The labeling scheme was slightly changed to fit the whole label on the tubes.

Fluorimeter Procedure

Imaging set-up



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your own words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Collection and Analysis

Images of High, Low, and Zero Calf Thymus DNA


Calibrator Mean Values


TABLE GOES HERE


Calibration curves


Images of Our PCR Negative and Positive Controls


PCR Results: PCR concentrations solved

TABLE GOES HERE


PCR Results: Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL


Observed results

  • Patient _____ :
  • Patient _____ :


Conclusions

  • Patient _____ :
  • Patient _____ :