BME100 s2017:Group3 W1030AM L5: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
<!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter --> | <!-- INSTRUCTIONS: In the space below, in your own words write the steps you performed to place samples onto the fluorimeter --> | ||
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# | # Collect and label all necessary samples. | ||
# | # Add 80 microliters of SYBR Green to the rougher side of the glass slide. It will appear as a bubble. | ||
# | # Discard the pipette tip and replace it. | ||
# Add 80 microliters of one of the Calf Thyme samples to the bubble of SYBR Green. | |||
# Adjust the glass slide so that the blue LED light shines through the bubble made in step 1 and 2 so that it hits the black fiber optic fitting. | |||
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Revision as of 12:30, 29 March 2017
BME 100 Spring 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportUsing new equipment for the first time was difficult but the pre-lab gave easy to understand instructions. It took some time for us to understand the difference between the first and second stop but by looking at the directions we were able to figure it out. The first stop takes in the liquid and the second stop releases it. Between the two stops we could feel a click within the pipette. Once we were done mixing the controls and patients tests there was a trace amount of DNA samples and PCR reaction mix left within their tubes. Because there was small varying amounts of liquid left of the DNA samples and PCR reaction mix the final reactions did not have exactly the same amount of liquid. With more practice using the pipette it would be easier to leave less amount of DNA samples and PCR reaction mix and have the same amount of liquid in the final reactions. During the experiment we did not have to change our labeling scheme, we used the same scheme that we planned to use. Fluorimeter ProcedureImaging set-up
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
Gel Electrophoresis
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