BME100 s2017:Group5 W8AM L4: Difference between revisions
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'''Background: About the Disease SNP''' | '''Background: About the Disease SNP''' | ||
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. --> | <!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. --> | ||
SNP, which stands for “single nucleotide polymorphism”, is a disease that causes a genetic alteration common in many individuals. The disease occurs when there is a single change in the nucleotide, or a building block of DNA consisting of Adenine, Thymine, Guanine, and Cytosine. On average, SNP is located around every 300 nucleotides. In our lab, we observed a variation of SNP located on the 7:117587799 chromosome. This specific SNP was linked to cystic fibrosis. | |||
"What Are Single Nucleotide Polymorphisms?" U.S. National Library of Medicine. National Institutes of Health, 21 Mar. 2017. Web. 21 Mar. 2017. | |||
Revision as of 21:06, 21 March 2017
BME 100 Spring 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
PCR Reaction Sample List
OpenPCR program
Research and DevelopmentPCR - The Underlying Technology Components on a PCR Reaction
Components during each step of the thermal cycling
Template DNA: Separates into two strands.
Template DNA: The two single strands tend to go back together but the abundance of primers in the tube prevents them of doing this.
Template DNA: DNA is now bind by the primers and Taq Polymerase, ready to be synthesized.
Template DNA: Each single-strand is synthesized in a copy of the original double stranded DNA molecule.
Template DNA: There is now two DNA molecules for each original template DNA at each PCR cycle, and the synthesized molecules are stabilized at the low temperature. Nucleotide Base Pairing
Base Pair Occurrence
SNP Information & Primer DesignBackground: About the Disease SNP SNP, which stands for “single nucleotide polymorphism”, is a disease that causes a genetic alteration common in many individuals. The disease occurs when there is a single change in the nucleotide, or a building block of DNA consisting of Adenine, Thymine, Guanine, and Cytosine. On average, SNP is located around every 300 nucleotides. In our lab, we observed a variation of SNP located on the 7:117587799 chromosome. This specific SNP was linked to cystic fibrosis. "What Are Single Nucleotide Polymorphisms?" U.S. National Library of Medicine. National Institutes of Health, 21 Mar. 2017. Web. 21 Mar. 2017.
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