BME100 s2017:Group6 W1030AM L5: Difference between revisions
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==PCR Reaction Report== | ==PCR Reaction Report== | ||
<!-- Write a summary of your team's experience with pipetting the samples to set up the reaction. Did the pre-lab reading help you? Did you understand the difference between the first and second stop on the pipettor? Did the final reactions have exactly the same amount of liquid? Was there any liquid left in the tubes that the DNA samples and PCR reaction mix? Did you have to change your labeling scheme? --> The pre-lab reading was very helpful. The videos show step by step how to use a pipette and tips for having successful transfers of liquids. When using a pipette, it is important to understand the difference between the first and second stop. The first stop on the pipette is used for retaining liquid. Once the desired amount has been set, place the pipette into the liquid and push down slowly to the first stop. This will allow the exact amount to be contained. The second stop is used to dispense the liquid and should be pressed down slowly as well. | <!-- Write a summary of your team's experience with pipetting the samples to set up the reaction. Did the pre-lab reading help you? Did you understand the difference between the first and second stop on the pipettor? Did the final reactions have exactly the same amount of liquid? Was there any liquid left in the tubes that the DNA samples and PCR reaction mix? Did you have to change your labeling scheme? --> | ||
The pre-lab reading was very helpful. The videos show step by step how to use a pipette and tips for having successful transfers of liquids. When using a pipette, it is important to understand the difference between the first and second stop. The first stop on the pipette is used for retaining liquid. Once the desired amount has been set, place the pipette into the liquid and push down slowly to the first stop. This will allow the exact amount to be contained. The second stop is used to dispense the liquid and should be pressed down slowly as well. | |||
==Fluorimeter Procedure== | ==Fluorimeter Procedure== |
Revision as of 11:05, 22 March 2017
BME 100 Spring 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportThe pre-lab reading was very helpful. The videos show step by step how to use a pipette and tips for having successful transfers of liquids. When using a pipette, it is important to understand the difference between the first and second stop. The first stop on the pipette is used for retaining liquid. Once the desired amount has been set, place the pipette into the liquid and push down slowly to the first stop. This will allow the exact amount to be contained. The second stop is used to dispense the liquid and should be pressed down slowly as well. Fluorimeter ProcedureImaging set-up
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved TABLE GOES HERE
PCR Results: Summary
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