BME100 s2017:Group7 W8AM L4: Difference between revisions
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'''Background: About the Disease SNP''' | '''Background: About the Disease SNP''' | ||
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. -- | <!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. --><br> | ||
A single nucleotide polymorphism (SNP) is a DNA sequence variation in one base pair which does not normally occur in other members of the same species. The specific SNP of interest for this laboratory has a reference code rs121908757, and specifically points to a SNP where an adenine nucleotide, in most of the species, becomes a cytosine nucleotide. This small and seemingly minute change in the genome pathogenic and affects the homo sapiens species by greatly increasing the chance of developing the disease cystic fibrosis. This genetic order most commonly causes damage to the lungs and also the digestive system. This SNP occurs on chromosome seven, results in approximately 70% of cystic fibrosis cases. | A single nucleotide polymorphism (SNP) is a DNA sequence variation in one base pair which does not normally occur in other members of the same species. The specific SNP of interest for this laboratory has a reference code rs121908757, and specifically points to a SNP where an adenine nucleotide, in most of the species, becomes a cytosine nucleotide. This small and seemingly minute change in the genome pathogenic and affects the homo sapiens species by greatly increasing the chance of developing the disease cystic fibrosis. This genetic order most commonly causes damage to the lungs and also the digestive system. This SNP occurs on chromosome seven, results in approximately 70% of cystic fibrosis cases. | ||
Revision as of 18:43, 21 March 2017
BME 100 Spring 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
The first step in the DNA sample setup is to take the extracted DNA and put a sample of it into the PCR tube. After this is complete, a sample of Primer 1 will then be taken from its capsule and put into the PCR tube along with the DNA sample. Now, Primer 2 will be added to the PCR tube which will eventually attach into the second site. Next, an excess amount of nucleotides will be added to the PCR tube in order to continue building DNA strands. The final component that will be added is the DNA Polymerase, an enzyme that will build back the strands of DNA using the surrounding nucleotides. Finally, the PCR tube will be placed into a DNA Thermal Cycler, which is a machine able to control the temperature of the PCR environment and dictate key changes in the reaction.
1) Set the DNA Thermal Cycler to 100°C
4) The sample is then kept at 72°C for 2 minutes
Research and DevelopmentPCR - The Underlying Technology Component Functions Thermal Cycling Process DNA nucleotides Base Pairing Occurrence
SNP Information & Primer DesignBackground: About the Disease SNP
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