BME103: Difference between revisions
Line 63: | Line 63: | ||
===Week 3: Measuring DNA Using Fluorescence, 10/31/12=== | ===Week 3: Measuring DNA Using Fluorescence, 10/31/12=== | ||
[[Image:Waterdrop-BME103-Group2.png|200px|thumb|Negative control water droplet.<br>Photo credit: | [[Image:Waterdrop-BME103-Group2.png|200px|thumb|Negative control water droplet.<br>Photo credit: Wed. 9:30am Group 2]] | ||
Revision as of 15:44, 17 December 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help |
Introduction to Biomedical Engineering - Lab Part 2
GETTING STARTED
THE COURSE IN REVIEWSet-up: OpenPCR BuildingBefore this unit began, a group of ~10 upper level undergrads and graduate students assembled the OpenPCR machines. This was a great experience for the graduate students, and saved our Freshmen engineers the time and trouble of assembling the delicate pieces from scratch in a very limited amount of time. Thanks to our assembly team and Dr. Pizziconi's Design Studio team for your help! Week 1: Introduction - DNA as a Biomarker, 10/17/12Students were introduced to basic DNA science and its relationship to diagnostics and health. Team members chose roles as Open PCR machine tester, Experimental protocol planner, and Research and development scientist.
In concurrent work sessions...
The principle that was taught was sequence-specific DNA hybridization. Primers designed to base-pair with a target disease-associated marker would allow exponential amplification of DNA, while a mismatch (non-disease DNA sequence) would not. Week 2: DNA Amplification Reactions, 10/24/12Students used their experience from the previous week to set up and run a PCR experiment. The students were provided with personal protective equipment, PCR reaction mix, diluted template + primers. The instructors set up positive and negative "patient" samples so that some samples would test positive for a DNA marker (produce amplification), and others would test negative They were introduced to a fluorescence-based DNA detection device that was designed by Dr. Garcia.
Activities:
Week 3: Measuring DNA Using Fluorescence, 10/31/12
|