BME103:Logistics: Difference between revisions
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** 3 Lab sections, ~85 students per section | ** 3 Lab sections, ~85 students per section | ||
*** 10 - 17 groups, of ~5 students per group | *** 10 - 17 groups, of ~5 students per group | ||
* 3 hours per | * 3 hours per class meeting | ||
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* [[BME103:TEMPwu1 | Lab Write-up 1]] | * [[BME103:TEMPwu1 | Lab Write-up 1]] | ||
* [[BME103:TEMPwu2 | Lab Write-up 2]] | * [[BME103:TEMPwu2 | Lab Write-up 2]] | ||
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'''MATERIALS & METHODS - DNA AMPLIFICATION''' | |||
* Reactions (100 μL total volume) contained: | |||
** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127]) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid | |||
** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence. | |||
** 2x GoTaq colorless PCR Master Mix - Promega M7133 | |||
* A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/ASU_courses/2012/10/24]). All other samples were measured via SYBRgreen staining on an LED fluorimeter. | |||
* All reactions were run on an [http://openpcr.org/ Open PCR machine]. | |||
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'''MATERIALS & METHODS - DNA IMAGING''' | |||
* Details to be added soon | |||
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Latest revision as of 16:12, 2 January 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help |
COURSE SPECS
TEMPLATE PAGES
MATERIALS & METHODS - DNA AMPLIFICATION
MATERIALS & METHODS - DNA IMAGING
OBTAINING MATERIALS
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