BME103:Logistics - Revision history

Karmella Haynes at 23:12, 2 January 2013

2013-01-02T23:12:06Z

←Older revision		Revision as of 23:12, 2 January 2013
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	'''MATERIALS & METHODS - DNA AMPLIFICATION'''		'''MATERIALS & METHODS - DNA AMPLIFICATION'''
	* Reactions (100 μL total volume) contained:		* Reactions (100 μL total volume) contained:
-	** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid	+	** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127]) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
	** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.		** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
	** 2x GoTaq colorless PCR Master Mix - Promega M7133		** 2x GoTaq colorless PCR Master Mix - Promega M7133

2013-01-02T23:11:36Z

←Older revision		Revision as of 23:11, 2 January 2013
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	** 3 Lab sections, ~85 students per section		** 3 Lab sections, ~85 students per section
	*** 10 - 17 groups, of ~5 students per group		*** 10 - 17 groups, of ~5 students per group
-	* 3 hours per ~~lab~~	+	* 3 hours per class meeting
	<br>		<br>

2013-01-02T23:10:58Z

←Older revision		Revision as of 23:10, 2 January 2013
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	'''MATERIALS & METHODS - DNA IMAGING'''		'''MATERIALS & METHODS - DNA IMAGING'''
	* Details to be added soon		* Details to be added soon
		+	<br>

	'''OBTAINING MATERIALS'''		'''OBTAINING MATERIALS'''

2013-01-02T23:10:31Z

←Older revision		Revision as of 23:10, 2 January 2013
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	* [[BME103:TEMPwu2 \| Lab Write-up 2]]		* [[BME103:TEMPwu2 \| Lab Write-up 2]]
	<br>		<br>
		+
		+	'''MATERIALS & METHODS - DNA AMPLIFICATION'''
		+	* Reactions (100 μL total volume) contained:
		+	** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
		+	** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
		+	** 2x GoTaq colorless PCR Master Mix - Promega M7133
		+	* A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/ASU_courses/2012/10/24]). All other samples were measured via SYBRgreen staining on an LED fluorimeter.
		+	* All reactions were run on an [http://openpcr.org/ Open PCR machine].
		+	<br>
		+
		+	'''MATERIALS & METHODS - DNA IMAGING'''
		+	* Details to be added soon

	'''OBTAINING MATERIALS'''		'''OBTAINING MATERIALS'''

2012-12-18T23:33:36Z

←Older revision		Revision as of 23:33, 18 December 2012
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-	'''COURSE ~~SIZE~~'''<br>	+	'''COURSE SPECS'''<br>
	* ~230 Freshmen		* ~230 Freshmen
-	* 3 Lab sections, ~85 students per section, 10 - 17 groups of ~5 students per ~~section~~	+	** 3 Lab sections, ~85 students per section
		+	*** 10 - 17 groups, of ~5 students per group
	* 3 hours per lab		* 3 hours per lab
	<br>		<br>
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	* [[BME103:TEMPwu1 \| Lab Write-up 1]]		* [[BME103:TEMPwu1 \| Lab Write-up 1]]
	* [[BME103:TEMPwu2 \| Lab Write-up 2]]		* [[BME103:TEMPwu2 \| Lab Write-up 2]]
-	* ~~[[BME103:TEMPwu3 \| Lab Write-up 3]]~~	+	<br>
		+
		+	'''OBTAINING MATERIALS'''
		+	* We have written several worksheets and hand outs for this course.
		+	* Please contact one of the instructors if you are interested in obtaining copies.


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2012-11-15T18:02:27Z

←Older revision		Revision as of 18:02, 15 November 2012
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	* [[BME103:TEMPwu1 \| Lab Write-up 1]]		* [[BME103:TEMPwu1 \| Lab Write-up 1]]
	* [[BME103:TEMPwu2 \| Lab Write-up 2]]		* [[BME103:TEMPwu2 \| Lab Write-up 2]]
		+	* [[BME103:TEMPwu3 \| Lab Write-up 3]]


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2012-10-31T07:30:10Z

←Older revision		Revision as of 07:30, 31 October 2012
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	'''COURSE SIZE'''<br>		'''COURSE SIZE'''<br>
	* ~230 Freshmen		* ~230 Freshmen
-	* 3 Lab sections, 85 students per section	+	* 3 Lab sections, ~85 students per section, 10 - 17 groups of ~5 students per section
	* 3 hours per lab		* 3 hours per lab
	<br>		<br>

2012-10-31T06:46:19Z

←Older revision		Revision as of 06:46, 31 October 2012
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	* 3 Lab sections, 85 students per section		* 3 Lab sections, 85 students per section
	* 3 hours per lab		* 3 hours per lab
		+	<br>

	'''TEMPLATE PAGES'''		'''TEMPLATE PAGES'''

2012-10-31T06:45:34Z

←Older revision		Revision as of 06:45, 31 October 2012
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	'''TEMPLATE PAGES'''		'''TEMPLATE PAGES'''
	* [[BME103:TEMPwu1 \| Lab Write-up 1]]		* [[BME103:TEMPwu1 \| Lab Write-up 1]]
		+	* [[BME103:TEMPwu2 \| Lab Write-up 2]]


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2012-10-25T22:21:16Z

←Older revision		Revision as of 22:21, 25 October 2012
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	'''TEMPLATE PAGES'''		'''TEMPLATE PAGES'''
-	* [BME103:TEMPwu1 \| Lab Write-up 1]	+	* [[BME103:TEMPwu1 \| Lab Write-up 1]]


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