BME103:Logistics

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
COURSE SPECS ~230 Freshmen 3 Lab sections, ~85 students per section 10 - 17 groups, of ~5 students per group 3 hours per lab TEMPLATE PAGES Lab Write-up 1 Lab Write-up 2 MATERIALS & METHODS - DNA AMPLIFICATION Reactions (100 μL total volume) contained: Template DNA - Kozak (BBa_BBa_J176012) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence. 2x GoTaq colorless PCR Master Mix - Promega M7133 A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [1]). All other samples were measured via SYBRgreen staining on an LED fluorimeter. All reactions were run on an Open PCR machine. MATERIALS & METHODS - DNA IMAGING Details to be added soon OBTAINING MATERIALS We have written several worksheets and hand outs for this course. Please contact one of the instructors if you are interested in obtaining copies.