BME103:T130 Group 1

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Tianzhu Zhu Experimental protocol planner Name: Wyatt Hansen Open PCR machine engineer Name: Bryce Hicok Open PCR machine engineer Name: Jesus Ibarra Experimental protocol planner Name: Emma Maiorella R&D scientist LAB 1 WRITE-UP Initial Machine Testing The Original Design The OpenPCR runs from your computer and connects through your USB port. You can add and delete steps, edit temperatures, create thermocycler protocols, from your computer. Experimenting With the Connections - When the PCB board of LCD is unlplugged from the Circuit Board, the LCD goes off. - When the white wire connecting the circuit board to the heat plate, the LCD showed an incorrect reading of the temperature. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction Polymerase Chain Reaction is the process of rapid duplication of a strand of DNA. The process first requires a DNA template, which contains the strand of DNA that is intended to be copied multiple times. The template strand is then been heated in order to separate the double stranded DNA and DNA Polymerase is added in order to create a strand of DNA that is complimentary to the original intended DNA strand, which creates a primer. Then the temperature is lowered so that the nucleotides will bind together in complementary to the primers in order to create a copy of the targeted DNA strand. The heating and cooling process will be repeated over a period of approximately 2 hours in order to create a large amount of DNA strand copies. Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)