BME103:T130 Group 10 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Frances Lakers Role: Protocol Planner Name: Tyler Tamasauckas Role: Protocol Planner Name: Jeffery Ramirez Role: R&D Specialist Name: Alexander Baldwin Role: Machine Design LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features There are two specific features that will be upgraded in the OpenPCR machine - the case and the power source. The case will be changed from a thin wood to a more durable plastic, so that the device can be taken out into the field. The device will also have solar panels, which will provide for the power. This shift allows the device to be used where electricity is not always available, such as rural and undeveloped regions. When combined, these two features open the device up to a larger market. Instructions The instructions would remain the same, as the solar panel array would come attached to the plastic on the device's top side. Protocols Materials Supplied in Kit Item Quantity PCR Machine 1 unit Camera Phone Stand 1 unit Fluorimeter Stand (LED Light) 1 unit BlackOut Box 1 unit Operator's Manual 1 unit Also included: Access code for smart phone app download. Supplied by User Item Quantity Smart Phone (w/ Camera) 1 unit USB/Phone Cord (if desired) 1 unit Micropipettes (as needed) Hydrophobic Glass Slides (as needed) Eppendorf Tubes Max 16 tubes/trial Sample DNA 0.2μL/tube; 16 tubes/trial GoTAQ Master Mix 50μL/tube; 16 tubes/trial 10 μM Forward Primer 1μL/tube; 16 tubes/trial 10 μM Reverse Primer 1μL/tube; 16 tubes/trial Distilled H2O 47.8μL/tube; 16 tubes/trial SYBR Green 0.1mL/test sample PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers The disease that we chose was Sickle Cell Anemia. Sickle Cell is a inherited disease that comes from both parents. If only one parent has the Sickle Cell trait, then the child will be a carrier. If both parents have the trait, then the child will have Sickle Cell. This disease causes the red blood cells to change into a crescent shape instead of being disk shape resulting in the loss of efficient oxygen delivery. It can also result in clotting because the blood cells can not make it through small blood vessels very well. The SNP for this disease is 78478128 and the reference SNP is rs78478128 Primer Design Illustration