BME103:T130 Group 10 l2
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
There are two specific features that will be upgraded in the OpenPCR machine - the case and the power source. The case will be changed from a thin wood to a more durable plastic, so that the device can be taken out into the field. The device will also have solar panels, which will provide for the power. This shift allows the device to be used where electricity is not always available, such as rural and undeveloped regions. When combined, these two features open the device up to a larger market.
The instructions would remain mostly the same, as the solar panel array would come attached to the plastic on the device's top side. The only changes in procedure would be in the use of an all in one phone application for the flourimeter measurements.
Also included: Access code for smart phone app download.
DNA Measurement Protocol
Research and Development
Background on Disease Markers
The disease that we chose was Sickle Cell Anemia. Sickle Cell is a inherited disease that comes from both parents. If only one parent has the Sickle Cell trait, then the child will be a carrier. If both parents have the trait, then the child will have Sickle Cell. This disease causes the red blood cells to change into a crescent shape instead of being disk shape resulting in the loss of efficient oxygen delivery. It can also result in clotting because the blood cells can not make it through small blood vessels very well. The SNP for this disease is 78478128 and the reference SNP is rs78478128.
The link to the page with more info on Sickle cell is http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001554/
The link to the page with the genetic information and SNP is http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=78478128#locus
The forward primer for Sickle Cell is 5'-tcctaagccagtgccagaag-3'
The reverse primer for Sickle Cell is 5'-gaattcgtctgtttcccattctaaac-3'
The mutation looks like this: TCCTGAGGAG with the bold a being the mutation.
A disease allele will give a PCR result and a non-disease allele will not because PCR is designed to amplify the area that corresponds to the mutation on the DNA. If it did not amplify only the target region, there would be too much information and a result would not be possible.