BME103:T130 Group 11 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name:Sharon Gooi Student Role: Experimental Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design The old design of the PCR Machine is as follows: The goal of our PCR redesign is to add more test tube space, making the PCR more efficient: In order to increase tube space, we increase the heating plate and increase the power proportionally to the size of the plate. We would also add more ventilation next to the heating plate in order to speed up the cooling process of the DNA. Key Features Instructions Protocols Materials Supplied in the Kit Amount SYBR Green I def Calf Thymus DNA ghi Pipettes jjk Microtubes gjk GoTAQ Master Mix jkh Fluorimeter jkhjh Fluroimeter stand and box lkj CD with ImageJ software jhkjh Supplied by User Amount DNA samples Smartphone for fluorimeter External computer Heatproof marker pen 1 PCR Protocol First, DNA samples were obtained from 31 different patients, each giving 3 DNA samples each. These DNA samples were stored in a freezer in microtubes. For patient number 1, his or her DNA samples were labelled 1a, 1b and 1c, using the heatproof marker. The other DNA samples from the remaining 30 patient were labelled in the same way. DNA Measurement Protocol Research and Development Background on Disease Markers The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16. Primer Design Illustration