BME103:T130 Group 11 l2

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BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
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OUR TEAM

Name:Sharon Gooi Student
Role: Experimental Protocol Planner
Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
The old design of the PCR Machine is as follows:


The goal of our PCR redesign is to add more test tube space, making the PCR more efficient:


Key Features


Instructions





Protocols

Materials


Supplied in the Kit Amount
SYBR Green I def
Calf Thymus DNA ghi
Pipettes jjk
Microtubes gjk
GoTAQ Master Mix jkh
Fluorimeter jkhjh
Fluroimeter stand and box lkj
CD with ImageJ software jhkjh


Supplied by User Amount
DNA samples
Smartphone for fluorimeter
External computer
Heatproof marker pen


PCR Protocol



DNA Measurement Protocol

Research and Development

The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 7.


Background on Disease Markers



Primer Design



Illustration