BME103:T130 Group 13 l2: Difference between revisions
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'''Background on Disease Markers''' | '''Background on Disease Markers''' | ||
<br><br>A particular single nucleotide polymorphism (SNP), rs132630309, is characterized by a mutated C instead of a normal T at position 5448 of the EDA (ectodysplasin A) gene on the X chromosome. The normal DNA sequence around this position is: while the SNP associated DNA sequence is:. At the protein level, this mutation causes a change from arginine (R; associated with the DNA triplet CGG) to leucine (L; associated with the DNA triplet CTG). The condition associated with this SNP is ectodermal dysplasia; this is a group of syndromes which is characterized by "abnormal development of the skin, hair, nails, teeth, or sweat glands" (Taken from the NCBI database). More info on this SNP can be found via this web link: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=132630309. | <br><br>A particular single nucleotide polymorphism (SNP), rs132630309, is characterized by a mutated C instead of a normal T at position 5448 of the EDA (ectodysplasin A) gene on the X chromosome. The normal DNA sequence around this position is: while the SNP associated DNA sequence is:. At the protein level, this mutation causes a change from arginine (R; associated with the DNA triplet CGG) to leucine (L; associated with the DNA triplet CTG). The condition associated with this SNP is hypohidrotic ectodermal dysplasia; this is one of a group of syndromes known as ectodermal dysplasia which is characterized by "abnormal development of the skin, hair, nails, teeth, or sweat glands" (Taken from the NCBI database). More info on this SNP can be found via this web link: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=132630309. | ||
<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) ---> | <!--- A description of the diseases and their associated SNP's (include the database reference number and web link) ---> |
Revision as of 19:40, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol Adjusting smartphone settings:
DNA Measurement Protocol After assembling the fluorimeter, you can now determine if you've amplified the targeted DNA in your PCR experiment. Using the Fluorimeter and the smartphone app, you can calculate the relative amount of DNA through fluorescence, which is generated by excitation and emission wavelengths. In order to detect fluorescence when dsDNA is present, you'll be using SYBR Green I because it's more safer compared to other dyes. With that being said, gloves must be worn when handling any liquid containing SYBR Green I. The fluorimeter itself is a very simple machine because it uses optical caustic, a special type of optics that completely removes the need for lasers, mirrors, or lenses. Also the flourimeter is battery-powered, lightweight and portable; this allows every student to have one of these at their lab table. Following the steps below, you can easily learn how to dye your amplified DNA.
1)On your lab table, you'll find eight samples from the Open PCR, 1 DNA sample(calf thymus standard at 2 micrograms/mL), and water from the scintillation vial (white cap) to analyze. Uploading Pictures and Analysis Using ImageJ
Research and DevelopmentBackground on Disease Markers
Illustration
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