BME103:T130 Group 14 l2 - Revision history

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:55:06 GMT

Research and Development

←Older revision		Revision as of 04:55, 30 November 2012
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	[[Image:Primer_design.jpg]]		[[Image:Primer_design.jpg]]
-		+	[[Image:Primer_d_2.jpg]]
	'''Illustration'''<br>		'''Illustration'''<br>

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:53:08 GMT

Research and Development

←Older revision		Revision as of 04:53, 30 November 2012
Line 222:		Line 222:


-		+	[[Image:Primer_design.jpg]]

	'''Illustration'''<br>		'''Illustration'''<br>
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	<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->		<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->

-	[[Image:~~Primer_design~~.jpg]]	+	[[Image:Illustration14.jpg]]
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

Nathaniel Bennett: /* Research and Development */

Nathaniel Bennett — Fri, 30 Nov 2012 04:49:56 GMT

Research and Development

←Older revision		Revision as of 04:49, 30 November 2012
Line 221:		Line 221:
	Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.		Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.

-	~~[[Image:Primer_design.jpg]]~~



-		+	'''Illustration'''<br>
-	'''Illustration'''	+

	<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->		<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->

-		+	[[Image:Primer_design.jpg]]
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

Nathaniel Bennett: /* Research and Development */

Nathaniel Bennett — Fri, 30 Nov 2012 04:49:08 GMT

Research and Development

←Older revision		Revision as of 04:49, 30 November 2012
Line 221:		Line 221:
	Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.		Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.

-	[[~~File~~:Primer_design.jpg]]	+	[[Image:Primer_design.jpg]]

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:45:52 GMT

Research and Development

←Older revision		Revision as of 04:45, 30 November 2012
Line 221:		Line 221:
	Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.		Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.

-	[File:~~Primerdesign~~.jpg]	+	[[File:Primer_design.jpg]]

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:35:02 GMT

Research and Development

←Older revision		Revision as of 04:35, 30 November 2012
Line 221:		Line 221:
	Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.		Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.

		+	[File:Primerdesign.jpg]

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:19:07 GMT

Research and Development

←Older revision		Revision as of 04:19, 30 November 2012
Line 219:		Line 219:

	<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->		<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
		+	Tay-Sachs disease is caused by a mutation of HEXA alleles on the 15th chromosome. People with the disease have a low or nonexistent B-hexo enzyme, allowing buildup of the GMD2 ganglioslide lipid.

Hope Haddad: /* Research and Development */

Hope Haddad — Fri, 30 Nov 2012 04:01:09 GMT

Research and Development

←Older revision		Revision as of 04:01, 30 November 2012
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	<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->		<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
		+	Tay-Sachs is a neural disease passed down from genetics. It can affect all age groups from infants to adults. You may be a carrier, but only two recessive traits will show symptoms of Tay-Sachs disease. The disease is caused by a defective gene on the 15th chromosome. Most signs are seen early on and can the disease can be tested for before birth. The affects of Tay-Sachs disease tend to work quickly. Some symptoms include loss of muscle function and strength, seizures, and/or blindness. Tay-Sachs disease in infants can kill a child by the age of four.

-		+	Tay-Sachs disease is associated with the SNP: Rs28940871
		+	http://www.ncbi.nlm.nih.gov/books/NBK22250/


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	<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->		<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
		+

Nathaniel Bennett at 22:00, 29 November 2012

Nathaniel Bennett — Thu, 29 Nov 2012 22:00:38 GMT

←Older revision		Revision as of 22:00, 29 November 2012
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	Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br>		Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br>
		+
		+	<br>
		+	{\| style="wikitable" width="800px"
		+	\|-
		+	\| [[Image:Fan_Group14.JPG\|370px]]
		+	\| [[Image:Heat_sink_Group14.JPG\|370px]]
		+	\|}
		+
		+	<br>
		+
	<br>		<br>
-	~~[[Image: Fan_Group14.JPG\|300px]][[Image: Heat_sink_Group14.JPG\|300px]]~~
-	~~<br><br>~~
	'''System Design'''<br>		'''System Design'''<br>
	Our redesign consisted of improving the insulation in the OpenPCR machine between the processing unit and fan and the the heating cap. This change is especially useful, allowing for increased insulation, with material such as fiberglass. This increased insulation allows for a more constant temperature at the heating cap. Because the temperature at the heating cap will be more constant, the OpenPCR machine will be able to perform its job faster and more efficiently, which is an incredibly handy feature considering a normal PCR machine can take up to two hours to amplify DNA. However, this increased insulation will cause the machine to become increasingly hot as it does its job. Our redesign accounts for this problem. By improving the fan on the machine as well, we eliminate any potential problems due to heat, such as overheating. However, another problem that arises from these new additions to the machine is room. The machine itself is fairly small, and compromising this portable size is not an option. For that reason, the redesign includes the use of a smaller fan, maintaining the intended efficiency of the redesign while accounting for the possible compromise in size. This redesign helps to improve the time that it takes for the OpenPCR machine to amplify DNA while maintaining a similar, if not smaller, size, allowing for much more DNA to be amplified in the same amount of time.		Our redesign consisted of improving the insulation in the OpenPCR machine between the processing unit and fan and the the heating cap. This change is especially useful, allowing for increased insulation, with material such as fiberglass. This increased insulation allows for a more constant temperature at the heating cap. Because the temperature at the heating cap will be more constant, the OpenPCR machine will be able to perform its job faster and more efficiently, which is an incredibly handy feature considering a normal PCR machine can take up to two hours to amplify DNA. However, this increased insulation will cause the machine to become increasingly hot as it does its job. Our redesign accounts for this problem. By improving the fan on the machine as well, we eliminate any potential problems due to heat, such as overheating. However, another problem that arises from these new additions to the machine is room. The machine itself is fairly small, and compromising this portable size is not an option. For that reason, the redesign includes the use of a smaller fan, maintaining the intended efficiency of the redesign while accounting for the possible compromise in size. This redesign helps to improve the time that it takes for the OpenPCR machine to amplify DNA while maintaining a similar, if not smaller, size, allowing for much more DNA to be amplified in the same amount of time.

Nathaniel Bennett at 21:57, 29 November 2012

Nathaniel Bennett — Thu, 29 Nov 2012 21:57:10 GMT

←Older revision		Revision as of 21:57, 29 November 2012
Line 26:		Line 26:
	Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br>		Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br>
	<br>		<br>
-	[Image: Fan_Group14.JPG]	+	[[Image: Fan_Group14.JPG\|300px]][[Image: Heat_sink_Group14.JPG\|300px]]
	<br><br>		<br><br>
	'''System Design'''<br>		'''System Design'''<br>