BME103:T130 Group 15: Difference between revisions
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# The 8 tubes of mixtures will then go through the cycles in the PCR system. | # The 8 tubes of mixtures will then go through the cycles in the PCR system. | ||
#* During each step of the thermal cycling, the DNA is unzipped and heated to 95°C to break the H-bonds between the 2 strands. This exposes the part we want in this lab experiment. Then, the primer binds to the trage we want without cancer marker, this primer won't bind. Next, the temperature will be dropped to 57°C in order to bind the primer. Later, it is heat it back up to 72°C with the Taq to reform and duplicate DNA strands. Finally, this thermal cycling is repeated for amplification and add dye that binds specifically to DNA for detection. | #* During each step of the thermal cycling, the DNA is unzipped and heated to 95°C to break the H-bonds between the 2 strands. This exposes the part we want in this lab experiment. Then, the primer binds to the trage we want without cancer marker, this primer won't bind. Next, the temperature will be dropped to 57°C in order to bind the primer. Later, it is heat it back up to 72°C with the Taq to reform and duplicate DNA strands. Finally, this thermal cycling is repeated for amplification and add dye that binds specifically to DNA for detection. | ||
< | <br> | ||
'''The Components of the GoTaq® Colorless Master Mix'''<br> | |||
"dNTP's, MgCl<sub>2</sub>, and reaction buffers at optimal concentration for efficient amplification of DN templates by PCR." | |||
<br> | |||
'''Volumes Used for Mixture'''<br> | |||
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|+ Table 1 | |+ Table 1 | ||
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The eight samples we ran in PCR were: <br> | The eight samples we ran in PCR were: <br> | ||
Patient ID, Gender, Ages <br> | Patient ID, Gender, Ages <br> |
Revision as of 15:10, 8 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine Testing
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction
The eight samples we ran in PCR were: Flourimeter Measurements (Add your work from Week 3, Part 2 here aka, steps of assembly to the flourimeter)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology There is a genetic relation to having cancer or not when an individual is over the age of 40. The specific gene, in this case, is located on chromosome 22, r17879961. To test an human's DNA for this cancer gene, we have go through a series of reactions called PCR on the DNA for replication and amplification of the patient's DNA strand.
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
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