BME103:T130 Group 1 l2: Difference between revisions
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'''Key Features'''<br> | '''Key Features'''<br> | ||
The key features of change we are focusing on is the casing and how it is assembled/dissembled. Our design changes are to use high density plastic instead of the wood casing currently used. We would also change from using screws to using a snap on feature. These simple changes will create major improvements in the PCR system. Using high density plastic will make it easy to see inside and to know when the heating lid is in proper place. It was also secure the safety issues of the wood being flammable. Then change from using screws to a snap on feature will make it easy and quick to assemble and disassemble. Before when using screws, it was stressful with dropping screws within the PCR causing you to have to start over with the assembly. With a snap on feature, the casing will simply snap on and off making the assembly quick and easy with no need of tools like a screwdriver. | |||
Revision as of 17:04, 27 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers The disease marker being used is SNP (single nucleotide polymorphism) rs35685286. This is the marker for sickle-cell disease found on chromosome 11. Patients with sickle-cell disease have red blood cells that are mishapen and are a "sickled" or crescent shape. This results in less oxygen being carried to the patient's body tissues. Therefore, patients experience crisis, where they have severe pain in their bones in their backs or chest. These symptoms can last for hours or even days. More information about this particular SNP can be found at: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35685286
Step A shows the PCR heating up to 95 degrees Celcius so the hydrogen bonds between the base pairs of the double stranded DNA can be melted and separated. Next in step B, the machine is cooled to 50 degrees Celcius where the reverse primers come in and attach to the single DNA strands to create two new double stranded DNA. Finally in step C, the PCR machine is heated up to 72 degrees Celcius and the strands are fully replicated and the process repeats for approximately 30 cycles. |