BME103:T130 Group 2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Alexandra Brunelle Research and Development Specialist Name: Charul Singh Experimental Protocol Planner Name: Alex Hoffmann Experimental Protocol Planner Name: AJ Ciferno Open PCR Machine Engineer Name: Haley Gjertsen Research and Development Specialist Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design Media:Open_PCR_Group_2.png Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction Reagent Volume Template DNA (20 ng) 0.2 µL 10 µM forward primer 1.0 µL 10 µM reverse primer 1.0 µL GoTaq master mix 50.0 µL dH20 47.8µL Total Volume 100.0 µL Sample Descriptions Sample 1: Positive Control (contains cancer DNA template), Tube label: P Sample 2: Negative Control (no DNA template), Tube label: N Patient 1 ID 74013, Male, Age 55 Sample 3: Patient 74013, Replicate 1. Tube label M1 Sample 4: Patient 74013, Replicate 2. Tube label M2 Sample 5: Patient 74013, Replicate 3. Tube label M3 Patient 2 ID: 72825, Female, Age 55 Sample 6: Patient 72825, Replicate 1. Tube label F1 Sample 7: Patient 72825, Replicate 2, Tube label F2 Sample 8: Patient 72825, Replicate 3, Tube label F3 Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)