BME103:T130 Group 3 l2: Difference between revisions
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'''Key Features'''<br> | '''Key Features'''<br> | ||
The specific feature being focused on is the heating and cooling aspect of the PCR machine. The main alteration of the machine would be to change the material of the heat sink and heating pad to copper. This would allow for a much quicker change in temperature whilst the machine is running, therefore the heating and cooling phases of the cycles would be attained faster thus cutting the time of a full PCR efficiently. | The specific feature being focused on is the heating and cooling aspect of the PCR machine. The main alteration of the machine would be to change the material of the heat sink and heating pad to copper. This would allow for a much quicker change in temperature whilst the machine is running, therefore the heating and cooling phases of the cycles would be attained faster thus cutting the time of a full PCR efficiently. The material change from a zinc alloy to copper would make heating and cooling the cycles of the PCR machine much quicker because copper is the most electrically conductive metal. | ||
Revision as of 16:37, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
ProtocolsMaterials
PCR Protocol 1.) Pipet 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20 in an Eppendorf tube. All of these items mixed together should create a total volume of 50.0μL. DNA Measurement Protocol Fluorimeter Setup Fluorimeter Measurements ImageJ Instructions Research and DevelopmentBackground on Disease Markers The single nucleotide polymorphism (SNP), 137852571, that is being examined in this experiment is linked with androgen insensitivity syndrome, and Kennedy spinal and bulbar muscular atrophy. Androgen insensitivity syndrome is when a person who is genetically male (who has one X and one Y chromosome) is resistant to male hormones (called androgens). As a result, the person has some or all of the physical traits of a woman, but the genetic makeup of a man. The mutation on the X chromosome makes the body unable to respond to the hormones that produce a male appearance. Kennedy spinal and bulbar muscular atrophy is a debilitating neurodegenerative disease resulting in muscle cramps and progressive weakness due to degeneration of motor neurons in the brain stem and spinal cord. The SNP is located on the X chromosome and affects the gene AR, the gene is inherited in an x-linked recessive manner therefore only males can be fully affected by the mutation and females are rarely affected. The sequence of this gene is:
Breast Cancer
Normal: G mutates into cancer A Illustration
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