BME103:T130 Group 4 l2: Difference between revisions
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# Clean off the first drop and repeat steps 4-5 using the next two rows of wells and the next sample to be tested. Up to 5 samples can be tested per slide. Obtain a new slide when all rows of wells have been used. | # Clean off the first drop and repeat steps 4-5 using the next two rows of wells and the next sample to be tested. Up to 5 samples can be tested per slide. Obtain a new slide when all rows of wells have been used. | ||
# In addition to your DNA samples, you will need to test a control sample of calf thymus and a water blank. Use the corresponding transfer pipette to place droplets of calf thymus in two adjacent middle wells on a glass slide and take photos as usual. Repeat the process with distilled water for your blank. | # In addition to your DNA samples, you will need to test a control sample of calf thymus and a water blank. Use the corresponding transfer pipette to place droplets of calf thymus in two adjacent middle wells on a glass slide and take photos as usual. Repeat the process with distilled water for your blank. | ||
# When all samples have been tested, transfer the photos from your smartphone to your computer and | # When all samples have been tested, transfer the photos from your smartphone to your computer. You will need to process a photo of each DNA sample as well as the calf thymus and water blank using Image J. | ||
# Open the desired photo in Image J. | |||
# In the menu, navigate to <b>Analyze>Set Measurements</b> and select <b>Area Integrated Density and Mean Gray Value</b>. | # In the menu, navigate to <b>Analyze>Set Measurements</b> and select <b>Area Integrated Density and Mean Gray Value</b>. | ||
# Then, navigate to <b>Image>Color>Split Channels</b>. This splits the image into Red, Blue, and Green files. Choose the Green one. | # Then, navigate to <b>Image>Color>Split Channels</b>. This splits the image into Red, Blue, and Green files. Choose the Green one. |
Revision as of 21:59, 23 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers The marker that is being used is rs137852453. This SNP is associated with hemophilia. Hemophilia is a condition where someone's blood is not clotting properly. Data on this particular SNP variance can be seen here: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=137852453
Bottom Primer (in reverse):
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