BME103:T130 Group 5 l2

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(Protocols)
(Protocols)
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'''PCR Protocol'''
'''PCR Protocol'''
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''' Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
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'' Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
-
'''
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''

Revision as of 17:35, 15 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Wade PatrickMachine Engineer
Name: Wade Patrick
Machine Engineer
Name: Liann KleinMachine Engineer
Name: Liann Klein
Machine Engineer
Name: Haylee PoncyProtocol Planner
Name: Haylee Poncy
Protocol Planner
Name: Kyle LabbanProtocol Planner
Name: Kyle Labban
Protocol Planner
Name: Alexandria LamR&D Scientist
Name: Alexandria Lam
R&D Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR Machine 1
Extra screws 5
CD containing programming application 1
Operations instruction manual 1
10 ft Extension cord 1


Supplied by the User Amount
Standard sized test tubes 16
DNA Primer Amounts vary per experiment
DNA Samples Amounts vary per experiment
Computer 1
Pipettes 16
Sybr Green Amounts vary per experiment
Refrigerator 1
Power source N/A

PCR Protocol

Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.


DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


Personal tools