BME103:T130 Group 5 l2: Difference between revisions
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==Research and Development== | <math></math>==Research and Development== | ||
<!--- Bonus: explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers ---> | <!--- Bonus: explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers ---> | ||
P(Alzheimer's/Positive)=P(Positive/Alzheimer's)P(Alzheimer's)/P(Positive) | |||
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<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. ---> | <!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. ---> | ||
The | The reverse primer is TAT TTT TAG A'''A'''G CGA TAA AA. The forward primer is GCC TCT GTT TTT TTC TCA GG. ONly a disease allele will have the sequence that allows the primers to bind. A non-diseased allele will not have it so primers will not bind. Taq polymerase will not know where to bind on the strand of DNA. Therefore, replication will not occur. | ||
Revision as of 21:42, 26 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
[math]\displaystyle{ }[/math]==Research and Development==
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