BME103:T130 Group 5 l2: Difference between revisions
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'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' | ||
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1. Collect samples generated from "PCR Protocol". | |||
2. Using separate pipettes for each individual sample, transfer the 150μL into the larger test tubes containing the proper solution. | |||
3. Using the fluorimeter equiptment, add two drops of each sample, followed by two drops of SYBR green. | |||
4. Close the system down, preventing any light from entering the system, and record a photo to visually measure the presence of a positive or negative result. | |||
5. Using a different pipette, clear the sample from the glass tray, move the tray forward, and repeat with the next sample. | |||
6. Continue until all samples have been measured and photographed. | |||
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==Research and Development== | ==Research and Development== |
Revision as of 15:41, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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