BME103:T130 Group 5 l2 - Revision history

Liann Kline: /* Thermal Cycler Engineering */

Liann Kline — Thu, 29 Nov 2012 16:33:04 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 16:33, 29 November 2012
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	'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]		'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]
-	<br> Eight wells were added, this is a good amount to increase sample size significantly yet not too drastic of a change that will affect the ~~machine completely~~.<br>	+	<br> Eight wells were added, this is a good amount to increase sample size significantly yet not too drastic of a change that will affect the size by making it too large.<br>

	[[Image:openpcrlid.png\|350px]]<br>		[[Image:openpcrlid.png\|350px]]<br>

Liann Kline: /* Thermal Cycler Engineering */

Liann Kline — Thu, 29 Nov 2012 16:27:41 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 16:27, 29 November 2012
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	'''Instructions'''<br>		'''Instructions'''<br>
-	The instructions for assembling the PCR machine will mostly stay the same the only difference will be that the ~~additional wells~~ will ~~cause the lid to~~ be ~~longer~~ than the original design.	+	The instructions for assembling the PCR machine will mostly stay the same the only difference will be that the screws will be located in a slightly different place than the original design.

	<!--- From Week 4 exercise --->		<!--- From Week 4 exercise --->

Liann Kline: /* Thermal Cycler Engineering */

Liann Kline — Thu, 29 Nov 2012 16:25:17 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 16:25, 29 November 2012
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	'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]		'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]
-	<br> Eight wells were added, this is a good amount to increase sample size significantly yet not to drastic of a change that will affect the machine completely.<br>	+	<br> Eight wells were added, this is a good amount to increase sample size significantly yet not too drastic of a change that will affect the machine completely.<br>

	[[Image:openpcrlid.png\|350px]]<br>		[[Image:openpcrlid.png\|350px]]<br>

Wade Patrick: /* Thermal Cycler Engineering */

Wade Patrick — Thu, 29 Nov 2012 15:56:35 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 15:56, 29 November 2012
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	[[Image:openpcrlid.png\|350px]]<br>		[[Image:openpcrlid.png\|350px]]<br>
-	The additional wells will also slightly elongate the lid	+	The additional wells will also slightly elongate the lid.

	'''Key Features'''<br> The major change of the PCR machine was that eight extra wells were added to increase the sample size from 16 to 24. With the addition of the extra wells, the machine will be able to run more samples. This also affects the size as the additional wells will cause it to be longer than the original design.		'''Key Features'''<br> The major change of the PCR machine was that eight extra wells were added to increase the sample size from 16 to 24. With the addition of the extra wells, the machine will be able to run more samples. This also affects the size as the additional wells will cause it to be longer than the original design.

Wade Patrick: /* Thermal Cycler Engineering */

Wade Patrick — Thu, 29 Nov 2012 15:55:02 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 15:55, 29 November 2012
Line 30:		Line 30:

	'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]		'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]
-	<br> 8 wells were added, this is a good amount to increase sample size significantly yet not to drastic of a change that will affect the machine completely.<br>	+	<br> Eight wells were added, this is a good amount to increase sample size significantly yet not to drastic of a change that will affect the machine completely.<br>

	[[Image:openpcrlid.png\|350px]]<br>		[[Image:openpcrlid.png\|350px]]<br>

Kyle Labban: /* Protocols */

Kyle Labban — Thu, 29 Nov 2012 04:49:25 GMT

Protocols

←Older revision		Revision as of 04:49, 29 November 2012
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	# Invert tubes, then turn upright to mix solution.		# Invert tubes, then turn upright to mix solution.
	# Plug in OpenPCR Machine and turn it on.		# Plug in OpenPCR Machine and turn it on.
-	# Open lid and place tubes into holder in PCR machine (the machine can now hold a maximum of 24 tubes).	+	# Open lid and place tubes into holder in PCR machine (the machine can now hold a maximum of 24 test tubes).
	# Close the lid.		# Close the lid.
	# Add the following cycles on the OpenPCR program:		# Add the following cycles on the OpenPCR program:
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	## Hold: 4 degrees		## Hold: 4 degrees
	# Run reaction.		# Run reaction.
-	# After the program is complete, open the lid and remove ~~tubes~~ for further analysis.	+	# After the program is complete, open the lid and remove samples for further analysis.

Kyle Labban: /* Protocols */

Kyle Labban — Thu, 29 Nov 2012 04:48:00 GMT

Protocols

←Older revision		Revision as of 04:48, 29 November 2012
Line 121:		Line 121:
	# Invert tubes, then turn upright to mix solution.		# Invert tubes, then turn upright to mix solution.
	# Plug in OpenPCR Machine and turn it on.		# Plug in OpenPCR Machine and turn it on.
-	# Open lid and place tubes into holder in PCR machine.	+	# Open lid and place tubes into holder in PCR machine (the machine can now hold a maximum of 24 tubes).
	# Close the lid.		# Close the lid.
	# Add the following cycles on the OpenPCR program:		# Add the following cycles on the OpenPCR program:

Kyle Labban: /* Protocols */

Kyle Labban — Thu, 29 Nov 2012 03:28:28 GMT

Protocols

←Older revision		Revision as of 03:28, 29 November 2012
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	'''PCR Protocol'''		'''PCR Protocol'''

-	~~'' Kyle - Create~~ a ~~step~~-~~by-step procedure for setting up and running PCR reactions~~. ~~Your instructions should include everything from adding reagents to~~ the tubes, to ~~programming the~~ PCR machine ~~and running~~ the reaction.	+	# Using a pipette, transfer 1.0-1.5 micro liters of the DNA sample into the desired number of test tubes.
-	''	+	# Pipette approximately 3 micro liters of reagent solution into each of the test tubes with the DNA.
		+	# Invert tubes, then turn upright to mix solution.
		+	# Plug in OpenPCR Machine and turn it on.
		+	# Open lid and place tubes into holder in PCR machine.
		+	# Close the lid.
		+	# Add the following cycles on the OpenPCR program:
		+	## Stage 1: 1 cycle, 95 degrees Celsius for 3 minutes
		+	## Stage 2: 30 cycles, 95 degrees for 30 seconds, 57 degrees for 30 seconds, 72 degrees for 30 second
		+	## Stage 3: 72 degrees for 3 minutes
		+	## Hold: 4 degrees
		+	# Run reaction.
		+	# After the program is complete, open the lid and remove tubes for further analysis.

Liann Kline: /* Thermal Cycler Engineering */

Liann Kline — Thu, 29 Nov 2012 00:44:48 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 00:44, 29 November 2012
Line 40:		Line 40:

	'''Instructions'''<br>		'''Instructions'''<br>
-		+	The instructions for assembling the PCR machine will mostly stay the same the only difference will be that the additional wells will cause the lid to be longer than the original design.

	<!--- From Week 4 exercise --->		<!--- From Week 4 exercise --->

Liann Kline: /* Thermal Cycler Engineering */

Liann Kline — Thu, 29 Nov 2012 00:40:09 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 00:40, 29 November 2012
Line 30:		Line 30:

	'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]		'''System Design'''<br> [[Image:BME103_group5_wells.jpg\|350px]]
-	<br> 8 wells were added<br>	+	<br> 8 wells were added, this is a good amount to increase sample size significantly yet not to drastic of a change that will affect the machine completely.<br>

	[[Image:openpcrlid.png\|350px]]<br>		[[Image:openpcrlid.png\|350px]]<br>
-	The additional wells will also elongate the lid	+	The additional wells will also slightly elongate the lid

	'''Key Features'''<br> The major change of the PCR machine was that eight extra wells were added to increase the sample size from 16 to 24. With the addition of the extra wells, the machine will be able to run more samples. This also affects the size as the additional wells will cause it to be longer than the original design.		'''Key Features'''<br> The major change of the PCR machine was that eight extra wells were added to increase the sample size from 16 to 24. With the addition of the extra wells, the machine will be able to run more samples. This also affects the size as the additional wells will cause it to be longer than the original design.