BME103:T130 Group 5 l2

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Contents

OUR TEAM

Name: Kyle LabbanProtocol Planner
Name: Kyle Labban
Protocol Planner
Name: Haylee PoncyProtocol Planner
Name: Haylee Poncy
Protocol Planner
Name: Liann KleinMachine Engineer
Name: Liann Klein
Machine Engineer
Name: Wade PatrickMachine Engineer
Name: Wade Patrick
Machine Engineer
Name: Alexandria LamResearch and Development Scientist
Name: Alexandria Lam
Research and Development Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR Machine 1
Extra screws 5
CD containing programming application 1
Operations instruction manual 1
10 ft Extension cord 1


Supplied by the User Amount
Standard sized test tubes 16
DNA Primer Amounts vary per experiment
DNA Samples Amounts vary per experiment
Computer 1
Pipettes 16
Sybr Green Amounts vary per experiment
Refrigerator 1
Power source N/A

PCR Protocol


Background on Disease Markers



Primer Design



Illustration


Personal tools