OUR TEAM
 Name: Wade Patrick Machine Engineer |  Name: Liann Klein Machine Engineer |  Name: Haylee Poncy Protocol Planner |  Name: Kyle Labban Protocol Planner |  Name: Alexandria Lam R&D Scientist |
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
| Supplied in the Kit
| Amount
|
| PCR Machine
| 1
|
| Extra screws
| 5
|
| CD containing programming application
| 1
|
| Operations instruction manual
| 1
|
| 10 ft Extension cord
| 1
|
| Supplied by the User
| Amount
|
| Standard sized test tubes
| 16
|
| DNA Primer
| Amounts vary per experiment
|
| DNA Samples
| Amounts vary per experiment
|
| Computer
| 1
|
| Pipettes
| 16
|
| Sybr Green
| Amounts vary per experiment
|
| Refrigerator
| 1
|
| Power source
| N/A
|
PCR Protocol
Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
DNA Measurement Protocol
1. Collect samples generated from "PCR Protocol".
2. Using separate pipettes for each individual sample, transfer the 150μL into the larger test tubes containing the proper solution.
3. Using the fluorimeter equiptment, add two drops of each sample, followed by two drops of SYBR green.
4. Close the system down, preventing any light from entering the system, and record a photo to visually measure the presence of a positive or negative result.
5. Using a different pipette, clear the sample from the glass tray, move the tray forward, and repeat with the next sample.
6. Continue until all samples have been measured and photographed.
Research and Development
Background on Disease Markers
Primer Design
Illustration
| 
BME 103 Fall 2012
