BME103:T130 Group 6: Difference between revisions
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<b>1.</b> The Polymerase Chain Reaction or PCR works by singling out a single piece of DNA and then multiplying it so there's millions of copies of one strand of DNA. It's a step by step process that first occurs by heating up the DNA to 100°C in order to denature the hydrogen bonds between the two strands of DNA so that both sides of the DNA can be accesible to copy. After the strands are separated, specific primers are added to locate the section of DNA to be amplified. Next, the Taq DNA polymerase is added which actually copies the section of DNA desired and synthesizes the second half of each strand. After this there are only a few copies of the DNA which is why the machine then replicates more strands by repeating the process multiple times until there are millions of copies. <br> | <b>1.</b> The Polymerase Chain Reaction or PCR works by singling out a single piece of DNA and then multiplying it so there's millions of copies of one strand of DNA. It's a step by step process that first occurs by heating up the DNA to 100°C in order to denature the hydrogen bonds between the two strands of DNA so that both sides of the DNA can be accesible to copy. After the strands are separated, specific primers are added to locate the section of DNA to be amplified. Next, the Taq DNA polymerase is added which actually copies the section of DNA desired and synthesizes the second half of each strand. After this there are only a few copies of the DNA which is why the machine then replicates more strands by repeating the process multiple times until there are millions of copies. <br> | ||
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<b>2. 1. collect DNA from patients <br> 2. Heat Denaturation- the sample is heated to break the bonds between the strands <br> 3.Primer Annealing- the solution cools and the DNA combines with the primer to copy the DNA | <b>2.</b> | ||
<ul> | |||
<li>1. collect DNA from patients</li> <br> | |||
<li>2. Heat Denaturation- the sample is heated to break the bonds between the strands</li> <br> | |||
<li>3.Primer Annealing- the solution cools and the DNA combines with the primer to copy the DNA the sample of DNA has been replicated.</li></ul> | |||
<br><br><b>3.</b> The GoTaq master mix contains 400µM dATP, 400µM dGTP, 400µM dCTP, 400µM dTTP, and 3mM MgCl<sub>2</sub> | <br><br><b>3.</b> The GoTaq master mix contains 400µM dATP, 400µM dGTP, 400µM dCTP, 400µM dTTP, and 3mM MgCl<sub>2</sub> | ||
<br><br><b>4.</b><center> | <br><br><b>4.</b> <center> | ||
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Revision as of 20:58, 12 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UP
Initial Machine TestingThe Original Design
When we unplugged the LED screen from the circuit board, the machine stopped displaying information on the LED screen. When we unplugged the white wire that connects the circuit board to the heating block, the heating block would not heat up.
(Write the date you first tested Open PCR and your experience(s) with the machine)
Protocols
Polymerase Chain Reaction
4.
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology
Results
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