BME103:T130 Group 6 l2: Difference between revisions
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8. Take a picture of the mixture on the fluorimeter (best done if camera is on a timer so that you can fully close the box and avoid excess light exposure. <br> | 8. Take a picture of the mixture on the fluorimeter (best done if camera is on a timer so that you can fully close the box and avoid excess light exposure. <br> | ||
9. Repeat steps 2-8 for each sample. <br> | 9. Repeat steps 2-8 for each sample. <br> | ||
<br> | |||
1. Download the Image J software<br> | |||
2. Open Image J<br> | |||
3. To chose your pictures click 'file' and 'open' then select the image you wish to analyze<br> | |||
3. Once your image is opened highlight and click on "analyze", "set measurements", and check the boxes "area" "integrated density" and "mean gray value" in order to just analyze the pieces for this lab.<br> | |||
4. After this you have to highlight and click "image", "color", then click "split channels" <br> | |||
5. This splits the image you have into three new images. For this assignment just keep the image "green" and disregard the others.<br> | |||
6. Then analyze the droplet in the picture to get the info for integrated density, mean gray value, and area. <br> | |||
7. Repeat steps for one picture of each sample. <br> | |||
8. Save your data in an excel page for easy access at another time<br> | |||
==Research and Development== | ==Research and Development== |
Revision as of 18:06, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||
OUR TEAMLAB 2 WRITE-UP
PCR Machine Improvements
Thermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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