BME103:T930 Group 11 l2: Difference between revisions
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'''PCR Protocol''' | '''PCR Protocol''' | ||
<br>Step 1: | <br>Step 1: Download the Open PCR Software onto Computer | ||
<br>Step 2: Open | <br>Step 2: Plug in and turn on the Open PCR machine, connect the USB cable to your computer | ||
<br>Step | <br>Step 3: On the machine interface, select "DNA replication" and then choose desired number of cycles (at least 30 for good results). | ||
<br>Step | <br>Step 4: Using the Pipette, transfer 32 samples of the patients DNA into each test tube. You should only use 1 Pipette tip for this part of the process. | ||
<br>Step 5: Next, transfer the forward and reverse primers into each of the 32 test tubes. 2 Pipette tips should be used in this part of the process: 1 for all of the forward primer transfers, and 1 for all of the reverse primer transfers. | |||
<br>Step 6: Using a new pipette tip, transfer the GoTaq Master mix into each of the 32 test tubes. | |||
<br>Step 7: Dilute the 32 solutions by filling the remainder of the test tube with distilled water. | |||
<br>Step 8: Carefully Label Each test tube with a sharpie making sure that the positive and negative controls are clearly marked. | |||
<br>Step 9: Open the heated lid of the Open PCR machine and place the test tubes into the designated slots. Close the lid. | |||
<br>Step 10: Make sure everything is properly connected and then choose "begin replication" on the interface. | |||
<br>Step 11: Check to make sure that the computer is correctly receiving the information, if not: stop the cycle, check the USB cord, and try again. | |||
Revision as of 20:52, 27 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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