When the heat sink is unplugged from the circuit board, the LCD screen turned off. When we unplugged the white wire that connected the circuit board to the heating block the temperature reading on the LCD screen dropped drastically.
Test Run
We first tested open PCR on October 18, 2012. We learned how to take accurate temperatures using the open PCR machine. Using open PCR we were able to make a polymerase chain reaction. In order for this to occur, open PCR had to send the DNA through different sets of temperatures to heat it up to separate the strands and expose the bases, cool it down for the primers to bind to the sequences, and also heat it back up so we can get an extension of the copy of the new DNA. Which was an hour and thirty minute long process.
Protocols
Polymerase Chain Reaction
Reagent
Volume
Template DNA (20ng)
0.2 μL
10 μM forward primer
1.0 μL
10 μM reverse primer
1.0 μL
GoTaq master mix
50.0 μL
dH2O
47.8 μL
Total Volume
100.0 μL
Here is the patient information:
92136 M 54 Years
62276 F 61 Years
Flourimeter Measurements
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
(Add a write-up of the information discussed in Week 3's class)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
(Your group will add the results of your Fluorimeter measurements from Week 4 here)
BME 103 Fall 2012
