BME103:T930 Group 16 l2

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(Thermal Cycler Engineering)
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'''Instructions'''<br>
'''Instructions'''<br>
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1) Take the pre-cut solar panels and insert them into the modified top of the open PCR machine.  
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1) Take the pre-cut solar panels and insert them into the modified top of the open PCR machine.<br>
-
2) Once inserted, let these solar panels charge under the light.  
+
2) Once inserted, let these solar panels charge under the light.<br>
-
3) To insert the crystalized monitor, screw it into the modified side of the open PCR machine. Before placing the monitor into the side of the PCR machine, make sure to connect the correct wires (directed by color) together in order for the monitor to operate.  
+
3) To insert the crystalized monitor, screw it into the modified side of the open PCR machine. Before placing the monitor into the side of the PCR machine, make sure to connect the correct wires (directed by color) together in order for the monitor to operate.<br>

Revision as of 13:58, 15 November 2012

BME 103 Fall 2012 Home
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Contents

OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
Image:group 16 top.png Image:group 16 side.png


Key Features
4 solar panels will be added to the top of the PCR machine. This will allow the PCR machine to run on its own without having to plug it into an outlet. Making the PCR, environmentally friendly, while still being able to detect different diseases in DNA samples. A crystalized monitor will also be placed on the PCR's side panel. The monitor will display the current status of the sample that is being used, along with the amount of time left in the process. Eliminating the necessity of a computer during this process.


Instructions
1) Take the pre-cut solar panels and insert them into the modified top of the open PCR machine.
2) Once inserted, let these solar panels charge under the light.
3) To insert the crystalized monitor, screw it into the modified side of the open PCR machine. Before placing the monitor into the side of the PCR machine, make sure to connect the correct wires (directed by color) together in order for the monitor to operate.


necessity



Protocols

Materials


PCR Protocol
Before start the experiment be sure that the solar panels on the PCR machine have been charged.


DNA Measurement Protocol



Research and Development

Background on Disease Markers



Primer Design



Illustration


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