BME103:T930 Group 1 l2: Difference between revisions

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'''PCR Protocol'''
'''PCR Protocol'''


1. You will need a PCR machine and computer. <br>
2. Download the PCR software onto the computer. <br>
3.





Revision as of 14:53, 28 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Nick Hool: Machine Engineer
Joseph Heath: Machine Engineer, R&D Scientist
Jessica Kemper: Protocol Planner
Maile Ravenkamp: Protocol Planner
Christian Boden: R&D Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR Machine 1
Positive Control DNA Sample 27282151
Negative Control DNA Sample 35526894
Patient 1 DNA Sample 19073943
Patient 2 DNA Sample 29391013
PCR: Patient 2 ID 11260 , rep 1 1903450
PCR: Patient 2 ID 11260, rep 2 5214727
PCR: Patient 2 ID 11260, rep 3 5099077


PCR Protocol

1. You will need a PCR machine and computer.

2. Download the PCR software onto the computer.

3.


DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration