BME103:T930 Group 2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR AWESOME TEAM Ryan Sullivan Research Development Scientist Miriam Acosta PCR Machine Engineer Ryan Keeney PCR Machine Engineer Juliana Ramos Experimental Protocol Planner Aaron Cornejo Experimental Protocol Planner LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design An Open PCR is a thermo-cycler which utilizes a heating plate and cooling fan to rapidly and precisely change the temperature of a DNA sample. The samples are placed in sample holder close to the heating plate. Using a computer program the PCR goes through a cycle of heat changes for a duration of time in order to facilitate the replication and amplification of the DNA sample. Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction ‎ ‎ Flourimeter Measurements Research and Development Specific Cancer Marker Detection - The Underlying Technology The r17879961 DNA was sequenced to determine the chain of nucleotides that it is composed of. A section of this DNA shows change in the allele sequence, which could indicate cancer. In the sequence, a change from "ATT" to "ACT" is the specific indication marker for cancer. The artificial primer that is introduced is meant to match up with the "ACT" and surrounding nucleotide sequence ("TGA"). When the primer attaches successfully, it allows the Taq polymerase to finish attaching nucleotides to the sequence, which will, ultimately, result in exponential DNA replication. If the primer does not attach successfully, then this means the person does not have a cancer-indicating sequence, and it will, therefore, not replicate the DNA exponentially. If the DNA replicates exponentially, then the fluorescent dye will show, and this would indicate a cancerous genome (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)