BME103:T930 Group 3 l2: Difference between revisions
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'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' | ||
1.) After your PCR has finished replication, take the samples to the fluorimeter. Using permanent marker number the transfer pipette at the bulb, so its only used for a single sample.<br> | |||
2.)With the permanent marker label the Eppendrof tubes at the top, you should have a total of 10 Eppendrof tubes labeled and 10 pipettes labeled.<br> | |||
3.)Transfer each sample separately into the Eppendorf tubes containing 400 ml of buffer.<br> | |||
4.)Using a specially labeled Eppendorf tube containing SYBR GREEN, with its own pippter, place two drops onto the first two center drops.<br> | |||
5.)Then using the sample place two drops on top of the SYBR GREEN solution drops.<br> | |||
6.)Then align the blue light of the fluorimeter so it passes through the drop.<br> | |||
7.)Then the smartphone operator should take a picture with the settings on the phone adjusted to inactive flash, iso to 800, white balance to auto, exposure to the highest setting and contrast to the lowest setting.<br> | |||
8.)This process should be repeated for all samples.<br> | |||
9.)Now your picture is ready for the Image J software.<br> | |||
==Research and Development== | ==Research and Development== |
Revision as of 14:45, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
DNA Measurement Protocol
1.) After your PCR has finished replication, take the samples to the fluorimeter. Using permanent marker number the transfer pipette at the bulb, so its only used for a single sample. Research and DevelopmentBackground on Disease Markers 1.) The amyloid Beta precursor protein for Alzheimer's - 2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy - Primer Design 1.) Forward primer: CTTC[G]ACCT
Illustration
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