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Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
Supplied in the kit
Amount
Open PCR Machine
1
Template DNA (20 ng)
0.2 μL
Fluorimeter
1
Open PCR Software
1
Image J Software
50
Glass Slides
50
Supplied by User
Amount
Pippets
8
Computer
1
10 μM reverse primer
1.0 μL
10 μM reverse primer
1.0 μL
GoTaq master mix
50.0 μL
dH2O
47.8 μL
PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
1.) The amyloid Beta precursor protein for Alzheimer's -
rs63751263 (http://omim.org/entry/104760#0008)
AGACGGAGGAGATCTCTGAAGTGAAG [A/C] TGGATGCAGAATTCCGACATGACTC