BME103:T930 Group 9: Difference between revisions
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=LAB 1 WRITE-UP= | =LAB 1 WRITE-UP= | ||
==Initial Machine Testing== | ==Initial Machine Testing== | ||
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==Results== | ==Results== | ||
<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA ---> | |||
<!--- Enter the values from your group's Data Analyzer table below. E6, F6, etc. are the excel cells from which you should copy your data. ---> | |||
{| {{table}} | |||
|- style="background:#f0f0f0;" | |||
| '''Sample''' || '''Integrated Density''' || '''DNA μg/mL''' || '''Conclusion''' | |||
|- | |||
| PCR: Negative Control || E6 || F6 || G6 | |||
|- | |||
| PCR: Positive Control || E7 || F7 || G7 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 1 || E8 || F8 || G8 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 2 || E9 || F9 || G9 | |||
|- | |||
| PCR: Patient 1 ID #####, rep 3 || E10 || F10 || G10 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 1 || E11 || F11 || G11 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 2 || E12 || F12 || G12 | |||
|- | |||
| PCR: Patient 2 ID #####, rep 3 || E13 || F13 || G13 | |||
|} | |||
KEY | |||
* '''Sample''' = <!--- explain what "sample" means ---> | |||
* '''Integrated Density''' = <!--- explain what "integrated density" means and how you did background subtraction to get this value ---> | |||
* '''DNA μg/mL''' = <!--- how you calculated this ---> | |||
* '''Conclusion''' = <!--- explain | |||
Revision as of 14:17, 9 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged part 3, the LCD, from part 6, the Open PCR circuit Board, the LCD on the machine turned off and no information appeared on the LCD screen.
Test Run On October 25 2012, we conducted our first test on our open PCR machine. We tested the machine to test the operation functionality. The initial test demonstrated the machine heat sink accuratly controlled and displayed the preprogrammed temperaute determined by the software on the computer. The overall successfullness of the machine was good, however it came with one difficulity, fluctuation of time to complete the preporgrammed cycles.
ProtocolsPolymerase Chain Reaction
Flourimeter Assembly Procedure
How to Open Pictures Using Image J
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology In Week 3 of our PCR experiment, we studied the cancer marker in the PCR experiment was correlated to the breast and colorectal cancer. Based on conditional probabilities, we found that the frequency of this cancer found in Finland was 7.8%. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.
Results
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